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. 2017 Jan 15;7(3):677–693. doi: 10.7150/thno.16357

Figure 6.

Figure 6

Transfection with PMMNCs-miR-100 (nano-miR-100) inhibits the proliferative ability of HN-13 cells. (A) Growth curves of HN-13 cells with indicated treatments, showing that nano-miR-100 transfection effectively inhibited the growth of HN-13 cells in culture. (B) BrdU incorporation assay showing that nano-miR-100 transfection significantly decreased the percentage of BrdU-positive HN-13 cells. (C) Flow cytometry-based cell cycle analysis showing that nano-miR-100 transfection significantly decreased the percentage of HN-13 cells in S-phase. (D) Representative images of BrdU assay. BrdU is labeled in green and miR-100 in red; nuclei were counterstained with DAPI (blue). Scale bar, 100 μm. (E) The representative images of flow cytometry-based analysis; the significantly decreased proliferation of the PMMNCs-miR-100-treated cells is reflected by the reduced shaded area of cell cycle S-phase. All error bars indicate standard deviation. N.S., no statistical signification; *, p<0.01 versus the control group.