Figure 3.

Example of albumin nanoparticles uptake by activated neutrophils and the migration of neutrophils to lung inflammation sites. (A) Fluorescence confocal microscopy of neutrophils from bronchoalveolar lavage fluid 2 h and 20 h after intravenous injection of Cy5-albumin NPs (red) (neutrophils were labelled by Alexa Fluor 488-labeled anti-mouse Gr-1 antibody, green). Nucleuses were stained by DAPI (blue). (B) PEG-coated NPs were not detected in the BALF while the albumin NPs were observed at 10 and 20 h. (C) Cy5-BSA NPs in BALF were not detected in the absence of neutrophil. Reprinted with permission from ref 73. Copyright 2015 American Chemical Society.