Fig. 3. S534A mice display increased expression of NF-κB–dependent genes and mortality.

(A and B) WT (n = 7) and S534A (n = 7) mice were injected i.v. with LPS (1 µg/kg) and sacrificed 4 hours later. (A) Liver tissue was then subjected to microarray analysis. The heatmap shows those genes that were differentially regulated in expression in the livers of LPS-treated S534A mice compared to those in the livers of LPS-treated WT mice (FDR <0.05). (B) Liver tissue from the indicated LPS-treated mice was subjected to qPCR analysis of the expression of the indicated NF-κB–dependent genes. Data are means ± SD of seven mice per genotype. (C) WT and S534A mice were injected i.v. with LPS (1 µg/kg) and then were sacrificed eight hours later. Liver RNA was extracted and subjected to qPCR analysis of the expression of the indicated NF-κB–dependent genes. Data are means ± SD of at least six mice per genotype. (D to F) WT (n = 13) and S534A mice (n = 14) were injected i.v. with LPS (20 mg/kg). (D) Survival was monitored for 96 hours. (E and F) Serum concentrations of TNF-α (E) and IL-1β (F) were determined by ELISA. Data are means ± SD of at least 12 mice per genotype and time point). *P < 0.05, **P < 0.01, ***P < 0.001.