Table 4.

Sums of fatty acids in total membrane phospholipids and in intramuscular triglycerides of the longissimus dorsimuscle expressed on a quantitative basis (mg/100gfresh tissue) of steers infused for 42 days with enteral-graded amounts of menhaden oil rich in LCn-3PUFA¹

	Treatment
Control oil %	4	2	0		Contrast P-value
Menhaden oil %	0	2	4	s.e.	Lin	Quad
Total phospholipids
LCn-3PUFA2	22.8	37.9	56.2	3.6	0.0001	0.71
Total n-3	23.9	39.0	59.2	3.8	0.0002	0.60
Total n-6	116.2	104.1	79.3	6.4	0.002	0.39
Saturated	105.1	100.1	104.7	2.9	0.93	0.16
Intramuscular triglycerides
LCn-3PUFA2	0.44	1.5	3.9	1.0	0.03	0.56
Total n-3	2.2	3.6	6.9	1.4	0.02	0.52
Total n-6	48.3	51.6	46.2	9.3	0.83	0.6
Saturated	406	464	506	66	0.16	0.9
Period effect on total fatty acid amount in intramuscular triglycerides (index of marbling) mg/100 g fresh tissue3
Period	1	2	3	s.e.	P
	515	1211	1435	210	0.02

Lin = linear effect of Menhaden oil amounts; Quad = quadratic effect of Menhaden oil amounts; LCn-3PUFA = long-chain n-3 polyunsaturated fatty acids; LS = least square.

¹LS means ± s; n = 6.

²LCn-3PUFA = 20:5n-3 + 22:5n-3 + 22:6n-3.

³LS means and P-value of the period term of the mixed procedure model.