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. 2017 Feb 9;13(2):912. doi: 10.15252/msb.20167386

Figure EV1. Overlap of pPDH and LEF1 spots in xenograft tumors.

Figure EV1

Convex hull image analysis of serial sections of SW480 mock xenograft tumors stained for pPDH and LEF1, as shown in Fig 1A and B, second panels.
  • A, B
    Isolated contour maps with convex hull outlines for pPDH and LEF1.
  • C–E
    pPDH and LEF1 contour maps were overlaid on each other and overlapped regions highlighted in blue. Different thresholds for spot detection were tested; each threshold condition revealed between 65 and 77% overlap between pPDH and LEF1 spots.
  • F
    Summary of overlap results.