Table 3.
Whole blood cell distribution and RANKL expression.
| Baseline | Follow-up | p value | |
|---|---|---|---|
| Neutrophils (%)a | 82 [71–91] | 90 [84–091] | 0.2662 |
| Neutrophils (×108 cells/L) | 12.7 [8.0–15.6] | 9.6 [8.4–12.9] | 0.2642 |
| RANKL+ neutrophils (%)b | 22 [3–41] | 53 [21–77] | 0.0856 |
| RANKL+ neutrophils (×108 cells/L) | 1.5 [0.3–4.3] | 5.9 [1.8–7.1] | 0.1475 |
| Neutrophil RANKL MFI | 33.2 [25.5–44.9] | 24.1 [21.7–28] | 0.0830 |
|
| |||
| T-cells (%)c | 62 [58–74] | 68 [52–72] | 0.5265 |
| T-cells (×108 cells/L) | 4.2 [2.4–5.2] | 3.4 [2.4–11.7] | 0.4131 |
| RANKL+ T-cells (%)b | 6.2 [0.8–24] | 6.7 [4.6–15.7] | 0.8984 |
| RANKL+ T-cells (×108 cells/L) | 0.30 [0.03–1.03] | 0.20 [0.16–0.69] | 0.7646 |
| T-cell RANKL MFI | 49 [41–55] | 32 [25–53] | 0.2061 |
|
| |||
| B-cells (%)c | 7.3 [4.8–14] | 9.2 [4.9–15.0] | 0.7364 |
| B-cells (×108 cells/L) | 0.40 [0.18–0.94] | 0.44 [0.23–1.51] | 0.9658 |
| RANKL+ B-cells (%) | 4.7 [2.0–6.7] | 14 [3–28] | 0.0088∗∗ |
| RANKL+ B-cells (×108 cells/L)b | 0.02 [0.01–0.06] | 0.06 [0.02–1.22] | 0.0029∗∗,† |
| B-cell RANKL MFI | 48 [38–80] | 30 [25–63] | 0.0401∗ |
Flow cytometry results are shown as median and interquartile range; agated on granulocytes from whole blood; bgated on the correspondent parent gate (neutrophil, T- or B-cell); cgated on the nongranulocyte cells from whole blood (also called the “monolymph” gate). RANKL: receptor activator of NF-κβ ligand; MFI: median fluorescence intensity (arbitrary units); ∗p < 0.05, ∗∗p < 0.01. †Remained significant after correction for multiple comparisons.