Table 2.

Linearity, accuracy, and precision data of tenofovir analysis

Linearity data of tenofovir calibration sets
Standard curve	Range (µg/mL)	Calibrators	Slope	y-Intercept	R2 value
Validation 1	1.0–10	6	0.0566	−0.0691	0.9995
Validation 2	1.0–10	6	0.0579	−0.0948	0.9996
Validation 3	1.0–10	6	0.0603	−0.0166	0.9995
Accuracy and precision: drug substance (n = 6)
Validation parameters	Validation solutions	1 µg/mL	2 µg/mL	6 µg/mL	10 µg/mL
Accuracy (%)	Set 1	94.6	96.8	101.2	99.4
	Set 2	95.5	98.6	100.0	99.5
	Set 3	98.0	99.1	100.7	99.8
Precision (%RSD)	Set 1	2.58	1.51	0.625	1.15
	Set 2	0.84	1.17	0.334	0.49
	Set 3	0.98	1.61	0.72	1.70

Abbreviation: RSD, relative standard deviation.