Table 3.
Parameters | Change | Retention time | Capacity factor (k’) | Symmetry | Area | Resolution | Plates (half width), m |
---|---|---|---|---|---|---|---|
Nominal condition | 4.055 ± 0.092 | 0.674 ± 0.225 | 0.668 ± 0.744 | 218.63 ± 0.152 | 15.748 ± 0.497 | 31,804 ± 1.122 | |
Flow rate, mL/min | (Low) 0.975 | 4.149 ± 0.158 | 0.713 ± 0.414 | 0.671 ± 0293 | 224.4 ± 0.293 | 15.838 ± 0.092 | 32,472 ± 0.091 |
(High) 1.025 | 3.966 ± 0.065 | 0.637 ± 0.209 | 0.666 ± 0.551 | 213.58 ± 0.069 | 15.652 ± 0.269 | 31,360 ± 1.226 | |
Injection volume, µL | (Low) 7.5 | 4.314 ± 0.085 | 0.780 ± 0.170 | 0.710 ± 0.928 | 161.113 ± 0.203 | 15.352 ± 0.419 | 35,811 ± 1.468 |
(High) 12.5 | 4.326 ±0.14 | 0.785 ± 0.253 | 0.716 ± 0.293 | 272.5 ± 0.231 | 15.166 ± 0.318 | 34,952 ± 1.673 | |
Temperature, °C | (Low) 24 | 3.897 ± 0.462 | 0.612 ± 0.419 | 0.680 ± 0.365 | 201.6 ± 0.275 | 15.173 ± 0.397 | 31,526 ± 0.306 |
(High) 28 | 3.865 ± 0.076 | 0.595 ± 0.203 | 0.688 ± 0.704 | 202.733 ± 0.138 | 15.108 ± 0.591 | 32,442 ± 1.395 | |
Organic phase, % | (Low) 4.9 | 4.082 ± 0.164 | 0.685 ± 0.430 | 0.660 ± 0.606 | 224.9 ± 0.275 | 15.807 ± 0.336 | 30,072 ± 1.328 |
(High) 5.1 | 3.89 ± 0.068 | 0.605 ± 0.171 | 0.655 ± 0.648 | 217.7 ± 0.369 | 15.493 ± 0.362 | 29,410 ± 0.134 | |
pH | (Low) 7.3 | 3.799 ± 0.060 | 0.568 ± 0.133 | 0.632 ± 0.527 | 234.0 ± 0.307 | 14.139 ± 0.396 | 27,362 ± 1.247 |
(High) 7.5 | 3.475 ± 0.148 | 0.434 ± 0.553 | 0.671 ± 0.523 | 233.8 ± 0.349 | 16.391 ± 0.484 | 28,403 ± 1.974 |