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. 2016 Sep 12;28(3):823–836. doi: 10.1681/ASN.2015080898

Figure 7.

Figure 7.

Functional characterization of MAP3K14 actions on cultured proximal tubular cells: chemokine expression. (A) MAP3K14 siRNA silencing in cultured murine proximal tubular cells suppressed MAP3K14 protein expression. Representative Western blot. (B) MAP3K14 siRNA silencing in cultured murine proximal tubular cells suppressed MAP3K14 mRNA expression. (C) MAP3K14 siRNA silencing prevents CXCL10 mRNA upregulation induced by a 24-hour stimulation by the noncanonical NFκB activator TWEAK (100 ng/ml). qRT-PCR. *p<0.01 versus control; **p<0.01 versus TWEAK alone. (D) MAP3K14 siRNA silencing prevents the increase in culture supernatants of the CXCL10 chemokine induced by exposure for 24 hours to 100 ng/ml TWEAK (ELISA). *p<0.001 versus control; **p<0.01 versus TWEAK alone. (E) MAP3K14 siRNA silencing prevents MCP1 mRNA upregulation induced by the noncanonical NFκB activator TWEAK. qRT-PCR. *p<0.001 versus scrambled; **p<0.001 versus TWEAK alone. (F) MAP3K14 siRNA silencing prevents RANTES mRNA upregulation induced by TWEAK. qRT-PCR. *p<0.002 versus scrambled; **p<0.003 versus TWEAK alone. Cells were treated with scramble or MAP3K14 siRNA before addition of 100 ng/ml TWEAK for 24 hours; mean±SD of three independent experiments.