Figure 1.

O. formigenes CM stimulates oxalate uptake by C2 cells. (A) C2 cells were UT or were treated apically with OM or CM in the culture medium (1:50 dilution × 24 hours), and then ¹⁴C-oxalate uptake was measured as described in the Concise Methods. Values are means±SEM of 12 independent experiments each of which was done in triplicate. The CM significantly stimulated ¹⁴C-oxalate uptake (*P<0.001 for CM compared with UT and OM, by ANOVA). (B) C2 cells were UT or were treated apically with L. acidophilus growth medium (MRS) or CM in the culture medium (1:25 dilution × 24 hours), and then ¹⁴C-oxalate uptake was measured as described in the Concise Methods. Values are means±SEM of three independent experiments each of which was done in triplicate. L. acidophilus CM had no significant effect on ¹⁴C-oxalate uptake by C2 cells.