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. 2016 Sep 9;28(3):761–768. doi: 10.1681/ASN.2016040486

Figure 2.

Figure 2.

Tnfr1-, Tnfr2-, and Tnfr1/2-deficient mice are protected from hyperoxaluria-related CKD and nephrocalcinosis. C57BL/6 wild type and Tnfr1-, Tnfr2-, and Tnfr1/2-deficient mice were fed either high oxalate or control diet for 14 days. (A) Plasma BUN and (B) plasma creatinine were measured. (C–F) Quantification of (C) tubular injury, (D) SMA+ area, (E) F4/80+ staining for macrophages, and (F) CD3+ cells per high power field. (G) Diagnostic imaging was performed using computed tomography. Arrows indicate the kidneys filled with CaOx crystals. (H) Pizzolato staining of kidney sections. Note that kidneys of wild-type mice, but not Tnfr1-, Tnfr2-, and Tnfr1/2-deficient mice fed with a high oxalate diet show CaOx monohydrate crystal deposition. Original magnification ×25. Data are means±SEM from six to seven mice in each group. ***P<0.001.