Figure 4.

The TNFR antagonist R-7050 protects hyperoxaluric mice from nephrocalcinosis and subsequent CKD. C57BL/6 male mice were fed high oxalate diet for 14 days with or without R-7050 (12 mg/kg intraperitoneally, every alternate day) treatment. (A) Gene expression was analyzed for CD44 and annexin II. (B) Quantification and (C) immunostaining for CD44, annexin II, and Pizzolato staining for CaOx. Original magnification ×25. (D) Plasma BUN and (E) plasma creatinine were measured. (F) Quantification of tubular injury. Data are means±SEM from six to eight mice in each group. *P<0.05; **P<0.01 versus vehicle group.