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Two-column purification process analyzed by SDS-PAGE and Western blot. (A) Coomassie-stained SDS-PAGE gel, (B) Western blot analysis using an anti-4xHis antibody, and (C) Western blot analysis using an anti-RuBisCO antibody. M: Molecular weight marker, Lane 1: E fraction from IMAC, Lane 2: FT fraction from Q column, Lane 3: E fraction from Q column.
