Figure 3.

Expression of the E7*-CP proteins in HEK-293 cells upon transfection. A: Immunoblotting of proteins from lysates of 1×10⁶ cells transfected with the E7*-CP fusions with or without ss. 1) Biotinylated Protein Ladder NEB; 2) E7* (cell lysate); 3) E7*-CP (cell lysate); 4) E7*-L-CP (cell lysate); 5) ss-E7* (cell lysate); 6) ss-E7*-CP (culture medium); 7) ss-E7 plant extract; 8) ss-E7*-L-CP (culture medium); 9) ss-E7*-CP (cell lysate); 10) ss-E7*-L-CP (cell lysate). B, C, D, E: Immunofluorescence (FITC) detected in cells transfected with E7*-CP (B, C) and E7*-L-CP (D,E) plasmids without or with the ss, respectively (original magnification, 100x; scale bar, 10 µm).