Figure 5.

Level of cytokines in culture supernatants from restimulated PCLS. PCLS were treated on day 2 of cultivation with either LPS, PMA/I, the 2010–2011 seasonal influenza vaccine (Flu) or tetanus toxoid (Tet) for 24 hours. After incubation, the level of cytokine in the culture supernatant was measured using the MSD Multi-Spot assay system: A) IFN-γ, B) TNF-α, C) IL-10 and D) IL-2. Values in the graphs represent the average pg/mL ± SD of PCLS from 4 different donor lungs normalized to a medium only control sample.