Figure 6.
The ratio of pDNA to HVJ-E. The ratio of pDNA to HVJ-E was investigated for the induction of the cytokine (IL-2). The ratio of 100 μg pDNA to 0.2∼2AU HVJ-E was administrated into C57BL/6 mice. HSP65 DNA + IL-12 DNA: pDNA 100μg + HVJ-E 0.2AU (), pDNA 100μg + HVJ-E 1AU (▪), pDNA 100μg + HVJ-E 2AU (
), control (5% trehalose phosphate buffer) (□). IL-2 production by the stimulation with 10 μg/ml of HSP65 protein or 20 μg/ml PPD antigen was analyzed by ELISA. * p<0.05: statistical significance (Student t).