Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 23;6(1):1267896. doi: 10.1080/20013078.2016.1267896

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and western blot (WB) analyses of UEV yield after hydrostatic filtration dialysis (HFD) and differential centrifugation. Ten μg of protein was loaded in each lane and stained with colloidal Coomassie (A). Lane 1 HFDa; Lane 2, HFDa-P40; Lane 3 HFDa-P200 g; Lane 4 HFDa-SN200. Western blots were probed with antibodies against: (B) dipeptidyl dipeptidase 4 (DDP4); (C) Tamm–Horsfall protein (THP); (D) neprilysin (NEP); (E) programmed cell death 6-interacting protein (ALIX); (F) tumour susceptibility gene 101 (TSG101); (G) tetraspanin CD63 (R&D Systems antibody); (H) tetraspanin CD81 (R&D Systems antibody); (I) tetraspanin CD9 (Merck Millipore antibody); (J) tetraspanin CD9 (R&D Systems antibody); (K) Anti ApoA1, (L) β-actin. Molecular weights are expressed in kilo Daltons (kDa).