Table 2.
Summary of the relative quantification in FLISA (blue), western blot (black), tuneable resistive pulse sensing (TRPS) and ELISA (ExoTESTTM). Values are expressed as percentage of the amount detected in HFDa [HFDa-X/HFDa × 100, where X is either P40 or P200 or SN200]. Red italic values are based on the ratio of the particle number in each fraction to the sum of the particles of all the fractions [HFDa-X/(HFDa-P40 + HFDa-P200 + HFDa-SN200) *100, where X is either P40 or P200 or SN200]. aR&D Systems antibody; bHansaBioMed antibody; cMerck Millipore antibody.
| HFDa-P40 | HFDa-P200 | HFDa-SN200 | ||||
|---|---|---|---|---|---|---|
| TSG101 | 16.4 | 35.9 | 12.8 | |||
| Alix | 65.4 | 62.9 | 16.2 | |||
| CD9a | 10.9 | 10.5 | 42.6 | 36 | 21.9 | 23.7 |
| CD9b | 10.2 | 1.4 | 47.2 | 108.4 | 8.2 | 25.0 |
| CD9c | 11.9 | 9.8 | 48.6 | 64.2 | 24.2 | 25.8 |
| CD63 a | 11.5 | 8.8/3.8 | 44.7 | 54.4/108.9 | 2.5 | 14.2/5.4 |
| CD63 b | 12.8 | 32 | 36.3 | 111 | 1.7 | 0.5 |
| CD81 a | 14.1 | 14.2 | 49.7 | 16.5 | 9.8 | 0.7 |
| CD81 b | 11.6 | 37.5 | 42.4 | 199.3 | 5.4 | 11.1 |
| Rab5 | 13.2 | 106.5 | 6.0 | |||
| FLOT 1 | 15.6 | 47.8 | 0.0 | |||
| TRPS | 85.2 50.7 | 66.3 33.6 | 46.8 15.7 | |||
| ExoTESTTM | 19.2 | 42.2 | 13.9 | |||