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In embryos cultured in SOF (A), 3iL (B), 5iLA (C) and NHSM (D) cells were identified by nuclear DAPI staining (blue) and the average cell number (±SD) was determined in day 8pf (E) and day 9pf embryos (F). Scale bar represents 50μm. Significant differences (p<0.05) are indicated by different letters.
