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. 2017 Feb 27;12(2):e0172920. doi: 10.1371/journal.pone.0172920

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© 2017 Brinkhof et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 7 — Relative expression levels of XIST (A) and HPRT1 (B) were determined by qRT-PCR in day 8pf embryos cultured in 3iL (blue), 5iLA (red) and NHSM (green) and compared with SOF day 8pf embryonic expression levels set at 1 (dashed line). Relative expression levels of XIST (C) and HPRT1 (D) in ICMs dissected from day 9pf embryos cultured in NHSM were obtained by microarray (mA; blue) and qRT-PCR (red) and compared with their SOF counterparts set at 1 (dashed line). Relative copy numbers (mean ± SD) of genomic SRY (E) was determined in parthenogenetically activated oocytes (OA), a combination of OA and IVF produced blastocysts cultured in SOF (green/blue), IVF SOF-cultured blastocysts alone (purple; set at 1), and cultured in NHSM (green) by PCR.