Table 1. Composition of culture media used.
| SOF | SOF + KOSR | NHSM | 3iL | 5iLA | Component | Function |
|---|---|---|---|---|---|---|
| * | * | KO-DMEM | TeSR1 | 1:1 DMEM/F12 & Neurobasal | Basic medium | |
| - | 20% | 20% | - | - | KOSR | |
| - | - | - | - | 1% | N2 supplement | |
| - | - | - | - | 2% | B27 supplement | |
| 4 mg/ml | 4 mg/ml | - | - | 50μg/ml | BSA | |
| - | - | 12.5 μ g/ml | - | - | Rec. Hum. Insulin | |
| 1% | 1% | 1% | - | 1% | NEAA 100x | |
| 2mM | 2mM | 1mM | - | 1mM | glutamine | |
| 2% | 2% | 0.1mM | - | 0.1mM | β-Mercaptoethanola | |
| 0.1% | 0.1% | 1% | - | 1% | pen/strep | |
| - | - | 20 ng/ml | 10 ng/ml | 20 ng/ml | Rec. Hum. LIF | |
| - | - | 8 ng/ml | - | - | Rec.bFGF | |
| - | - | 1 μ M | 1 μ M | 1 μ M | PD0325901 | MEK-inh |
| - | - | 10 μ M | - | - | SP600125 | JNK-inh |
| - | - | 10μM | - | - | SB203580 | p38-MAPK-inh |
| - | - | 5 μ M | - | - | Go6983 | PKC-inh |
| - | - | - | - | 0.5μM | SB590885 | B-Raf-inh |
| - | - | 3μ M | - | - | CHIR99021 | GSK3a/b-inh |
| - | - | - | - | 1 μ M | IM-12 | GSK3b-inh |
| - | - | - | 2 μ M | - | BIO | GSK3a/b-inh& JAK-inh |
| - | - | - | 2 μ M | - | Dorsomorphin | AMP kinase-inh& BMP-inh |
| - | - | 1 ng/ml | - | - | TGFβ1 | TGFβ- SMAD pathway |
| - | - | - | - | 20 ng/ml | Activin A | TGFβ- SMAD pathway |
| - | - | - | - | 1 μ M | WH-4-023 | Lck/Src-inh |
| - | - | - | - | 10 μ M | Y27632 | ROCK1-inh |
* details of SOF can be found in methods section.
a Different suppliers are used for SOF (SigmaAldrich) and NHSM or 5iLA (Life Technologies).