Table 3. Bovine ICM culture in 4 media and 2 coatings.
| Medium | Coating | ICMs | Colonies after 7 days | Viable at end of passage number (p) | ||||
|---|---|---|---|---|---|---|---|---|
| p1 | p2 | p3 | p4 | |||||
| 3iL | CT | 24 | 24 | 2 | 0 | |||
| 521/EC | 24 | 24 | 0 | |||||
| 5iLA | CT | 24 | 24 | 3 | 3 | 2 | 0 | |
| 521/EC | 24 | 24 | 6 | 2 | 0 | |||
| NHSM | CT | 24 | 24 | 0 | ||||
| 521/EC | 24 | 24 | 1 | 0 | ||||
| SOF | CT | 24 | 0 | |||||
| 521/EC | 24 | 0 | ||||||
ICMs were dissected from embryos cultured in 3iL, 5iLA, NHSM or SOF and plated in 24-well plates coated with either Cell-Tak (CT) or Laminin 521 / E-Cadherin (521/EC). After 7 days in their respective condition colonies were passaged.