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. Author manuscript; available in PMC: 2017 Feb 27.
Published in final edited form as: J Am Chem Soc. 2016 Dec 2;138(49):15861–15864. doi: 10.1021/jacs.6b10898

Figure 2.

Figure 2

Biochemically mediated interconversion of Mn-JED oxidation states by peroxidase and thiols. (A) Conversion of Mn2+-JED (top trace, 7.6 min) to Mn3+-JED (bottom trace, 6.1 min) triggered by H2O2/ peroxidase (25 U/mL) in PBS buffer monitored by HPLC with 254 nm detection. The difference in peak height between the pre- and post-peroxidase treated samples results from the 2-fold higher extinction co-efficient of Mn3+-JED. (B) Oxidation of Mn2+-JED in the presence of H2O2 (open circles) or H2O2/peroxidase (15 U/mL, closed circles) in human blood plasma monitored by NMR. H2O2 was generated in situ by the glucose/ glucose oxidase reaction. (C) Reduction of Mn3+-JED in human blood plasma without (open circles) and with 5 mol. equiv. L-Cys (filled circles) added. Relaxometry measurements were performed at 1.4T, 37 °C.