Alignment of the N gene region (positions 478 to 548) amplified by the MeVA RT-qPCR. The alignment includes examples of each genotype available on GenBank for this region, except for genotype D9, which was sequenced from one of our archival specimens. Row 1, 31 identical sequences from various vaccine strains; row 2, MVi/Maryland.USA/54 (A); row 3, two vaccine strains showing a 1-nt difference in the forward primer region; row 4, MVi/Yaounde.CMR/12.83 (B1); row 5, MVi/Libreville.GAB/84 (B2); row 6, MVi/Ibadan.NIE/971 (B3); row 7, MVi/New_York.USA/94 (B3); row 8, MVi/Maryland.USA/77 (C2); row 9, MVi/Illinois.USA/89/1 (D3); row 10, MVi/Montreal.CAN/89 (D4); row 11, Bankok.THA/12.93 (D5); row 12, MVi/New_Jersey.USA/94/1 (D6); row 13, MVs/Dundee.UNK/82 (D7); row 14, MVi/BritishColumbia.CAN/13.10/1 (D8); row 15, MVi/Manchester.GBR/30.94 (D8); row 16, MVs/Ontario.CAN/14.14 (D9); row 17, MVi/Berkeley.USA/83 (G1); row 18, MVi/Amsterdam.NLD/49.97 (G2); row 19, MVi/Gresik.IDN/17.02 (G3); row 20, MVi/Hunan.CHN/93/7 (H1); row 21, MVi/Beijing.CHN/94/1 (H2).