TABLE 1.
Most abundant immunoaffinity-purified proteins from TESA extracellular vesiclesa
| TESA protein | Accession no. | Mass (kDa) | No. of peptides in: |
|
|---|---|---|---|---|
| Nonimmune serum | Chagas immune serum | |||
| Retrotransposon | ||||
| RHS protein | K4E7X9 | 77 | 0 | 29 |
| Paraflagellar rod | ||||
| Paraflagellar rod protein 3 | K4EBQ5 | 70 | 0 | 18 |
| Paraflagellar rod protein 3 | K2MCT5 | 69 | 0 | 17 |
| Mitochondrial | ||||
| ATP synthase subunit alpha | K4E934 | 63 | 2 | 17 |
| ATP synthase subunit beta | K2M741 | 56 | 0 | 9 |
| Enoyl coenzyme A hydratase, mitochondrial | K4DTD3 | 29 | 0 | 8 |
| Malic enzyme | K4E9P4 | 63 | 0 | 6 |
| trans-Sialidase | ||||
| Putative uncharacterized protein | Q7YZX6 | 95 | 0 | 16 |
| trans-Sialidase, group VIII, putative | TcCLB.506537.200 | 120 | 0 | 15 |
| trans-Sialidase, putative (fragment) | K4DRD9 | 35 | 0 | 13 |
| trans-Sialidase | Q9BHJ5 | 71 | 0 | 12 |
| trans-Sialidase, putative | K4E110 | 81 | 0 | 12 |
| trans-Sialidase, putative (fragment) | K4DRD9 | 35 | 0 | 11 |
| trans-Sialidase | P23253 | 120 | 0 | 10 |
| trans-Sialidase, putative (fragment) | Q4D3D0 | 50 | 0 | 10 |
| Glycosomal | ||||
| Glycosomal membrane protein (PEX11) | K4DY43 | 27 | 0 | 8 |
| Fructose-bisphosphate aldolase | K4DLE5 | 41 | 0 | 7 |
| Tubulin | ||||
| β-Tubulin (pseudogene) | TcCLB.509003.70 | 33 | 0 | 6 |
| α-Tubulin | Q26973 | 47 | 0 | 6 |
| Glycotransferase | ||||
| UDP-Gal/DP-GlcNAc-dependent glycosyltransferase | Q4CS30 | 43 | 0 | 9 |
| Proteases | ||||
| Cytoskeleton-associated protein CAP5.5,cysteine peptidase | K4E5Y1 | 88 | 0 | 13 |
| Serine carboxypeptidase S28, putative | K4DZV5 | 54 | 0 | 6 |
| Heat shock protein | ||||
| Heat shock protein 70 | B5U6T4 | 71 | 0 | 3 |
| Uncharacterized | ||||
| Uncharacterized protein | K4EDD2 | 34 | 0 | 8 |
| Uncharacterized protein | K4EBS5 | 88 | 0 | 7 |
| Cytoskeleton protein | ||||
| Clathrin heavy chain | K4E050 | 193 | 0 | 12 |
| Sterol synthesis | ||||
| Lanosterol synthase, putative | K4EA17 | 103 | 0 | 9 |
| Endoplasmic rectiulum | ||||
| Pretranslocation protein, alpha subunit, SEC61-like | TcCLB.506297.240 | 54 | 0 | 8 |
| Glucose-regulated protein 78 | K4DT97 | 71 | 0 | 6 |
| Lysosomal | ||||
| Lysosomal α-mannosidase, putative | K4E2E6 | 111 | 0 | 7 |
| Acidocalcisome | ||||
| Vacuolar-type proton translocating pyrophosphatase 1 | K2NKA3 | 85 | 0 | 7 |
| Miscellaneous proteins | ||||
| Surface protein TolT, putative | Q4CPM8 | 32 | 0 | 5 |
a
Amino acid sequences for the proteins may be found in the NCBI Protein, Tritryp, and Uniprot/TrEMBL databases using the accession numbers listed in the table.