FIG 7.

MyD88 aids in systemic Brucella clearance and T cell IFN-γ production. C57BL/6 WT and MyD88^−/− mice were infected in each rear footpad with 1 × 10⁶ CFU of B. melitensis 16M. At days 1, 3, 7, 14, and 28, mice were euthanized, and bacterial loads were enumerated in the spleen (3 to 4 mice/group/time point) (A). At days 7 and 14, flow cytometry was performed to determine IFN-γ production by T cells from infected mouse spleens (B). (C) Representative plots showing IFN-γ production by gated CD4⁺ and CD8⁺ T cells from WT and MyD88^−/− mice 14 days postinfection. *, P < 0.05 compared to WT mice. Error bars depict standard errors of the mean. Data are representative of one kinetic experiment.