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IFA and confocal microscopy of oocyst production following infection with purified C. parvum sporozoites at various time points. (A to C) Oocysts within scaffolds; (E to G); oocysts within luminal material; (I to K) oocysts within culture medium; (D, H, L), uninfected controls. At each time point, scaffolds or material from the lumen or culture medium was fixed, permeabilized, and stained with an oocyst-specific MAb (Crypt-a-Glo).
