FIG 6.
Fap1 is required for binding of ATCC 10557 to β-1,4-linked galactose on platelets. (A) ATCC 10557 Δfap1 did not show further reduced binding to neuraminidase-pretreated (NPRE; 0.78 μM) platelets in the presence of SpBgaA146-990 (B; 0.054 μM), CBM71 (250 μM), or lactose (5 mM). Adherence is expressed as a percentage of the inoculum. Values are the means for three independent experiments, each performed in triplicate, ± SD. Statistical significance was tested by two-tailed Student's t test. *, P ≤ 0.03; NS, not significant. (B) Schematic representing domains predicted by Phyre2 within the nonrepeat region (NRR) of Fap1. Numbers refer to the amino acid positions within the stated protein. The horizontal lines denote the domains identified within GspB (PDB entry 3QC5) and CtCel9D-Cel44A (PDB entry 2C26) as sharing structural similarity with the nonrepeat region of Fap1. The vertical lines indicate the boundaries of the different domains. The percent identity and confidence in the Phyre2 prediction are shown on the right.