FIG 4.

G55, K58, and K59 are critical for recombinant AmOmpA to optimally bind to mammalian host cells. RF/6A cells were incubated with His-tagged AmOmpA or versions thereof in which specific residues were replaced with alanine. The cells were successively incubated with His tag antibody and Alexa Fluor 488-conjugated anti-mouse IgG and analyzed by flow cytometry. (A) Representative histograms (A) and the mean fluorescence intensities ± SD from triplicate samples (B) are presented. Data are representative of three independent experiments with similar results. Statistically significant (***, P < 0.001) values compared to AmOmpA are indicated.