FIG 8.
6-Sulfo-sLex is dispensable for recombinant AmOmpA binding to RF/6A cell surfaces and for A. marginale infection. (A) RF/6A cells were incubated with CSLEX1, KM93, G72, or IgM control for 1 h, followed by the addition of His-OmpA. Unbound recombinant protein was then washed away. Flow cytometry was used to detect bound His-AmOmpA. Cells alone served as a negative control. The histogram is representative of three independent experiments done in triplicate. (B and C) RF/6A endothelial cells were pretreated with IgM or G72. These cells were then incubated with DC A. marginale organisms for 2 h, after which unbound bacteria were removed. Cells were examined after 48 h by immunofluorescence microscopy to determine the percentage of infected cells (B) and AmVs per cell (C).