FIG 5.

Relative iNOS and cytokine expression in BMDMs. (A and B) Gene expression of iNOS measured in BMDMs infected with M. bovis wild type, mutant MbΔp27, or complemented strain MbΔp27::p27/p55. The relative gene expression was calculated using the 2^−ΔΔCT method with E correction, using GAPDH mRNA expression as the reference gene and uninfected cells as the calibrator. Asterisks indicate significance (*, P ≤ 0.05). The data were analyzed using a paired Student t test. (A) Replicates of the experiments. (B) Data expressed as a bar graph. (C, D, and E) Gene expression was measured in BMDMs infected with M. bovis wild type or mutant MbΔp27. The relative gene expression of IL-1β (C), IL-6 (D), or IL-12p35 (E) was calculated using the 2^−ΔΔCT method with E correction, using GAPDH mRNA expression as the reference gene and uninfected cells as the calibrator. Data were analyzed using a paired Student t test. ns, not statistically significant.