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. 2017 Feb 23;61(3):e01967-16. doi: 10.1128/AAC.01967-16

FIG 1.

FIG 1

Construction, expression, and purification of ZIKV NS5, NS5pol, and NS5mut. (A) Schematic diagram of the NS5, NS5pol, and NS5mut expression vectors. NS5mut contains an active-site mutation (GDD → GAA) in amino acids 663 to 665. MTase, methyltransferase domain. (B) SDS-PAGE analysis of protein expression and purification. Lanes T, total cell lysate; lanes S, soluble fraction; lanes E, eluate from the HisPur Ni-NTA agarose resin; lane M, protein standard marker. The sizes of protein markers (in kilodaltons) are indicated on the right.