FIG 5.

(A) Chemical structures of the ribonucleotide 5′-triphosphate analogs used in the assay. (B) Analysis of chain termination ability of ribonucleotide analogs in the reaction with ZIKV NS5 RdRp. The P/T duplex used in the assay is indicated at the top. The polymerase reactions were initiated by addition of the ribonucleotide analogs at 100 μM and of the three complementing natural ribonucleotides at 10 μM each, as indicated under each lane, and the products of the reactions were resolved by denaturing PAGE.