TABLE 1.

Sequences of labeled and unlabeled oligonucleotides used in the study

Oligonucleotide	Sequencea
Labeled RNA primer	Cy5.5-5′-AGAACCUGUUGAACAAAAGC-3′
Labeled DNA primer	Cy5.5-5′-AGAACCTGTTGAACAAAAGC-3′
RNA template 1	3′-UCUUGGACAACUUGUUUUCGGCUUAUUA-5′
DNA template	3′-TCTTGGACAACTTGTTTTCGGCTTATTA-5′
RNA template 2	3′-UCUUGGACAACUUGUUUUCGAGCUUAUUC-5′
Template for ATP analog K1/2	3′-UCUUGGACAACUUGUUUUCGAUCGUUAUUA-5′
Template for UTP analog K1/2	3′-UCUUGGACAACUUGUUUUCGCAGUUUUUU-5′
Template for GTP analog K1/2	3′-UCUUGGACAACUUGUUUUCGACUUAUUA-5′
Template for CTP analog K1/2	3′-UCUUGGACAACUUGUUUUCGUGCAUUAUUA-5′
Template for UTP analog IC50	5′-A30AACAGGUUCUAGAACCU GUU-3′

^aBoldface type indicates a single-stranded template part after P/T duplex formation.