FIG 1.
Characteristics of the P. aeruginosa fluorescent reporter strains. (A) Colonies of PAO1 and its isogenic ΔnfxB strain with the PCD-gfp+ reporter grown on LB agar plates and examined under an epifluorescence microscope with blue excitation light (green fluorescence) and green excitation light (red fluorescence). (B) The green fluorescent signal together with the OD600 was monitored during 24 h of planktonic growth of PAO1 and the ΔnfxB mutant with the PCD-gfp+ reporter. Raw fluorescence values were divided by the corresponding OD600 at each time point. The fluorescence of parent strains (resulting from the autofluorescence background) was subtracted from the fluorescence of reporter strains. The means and 95% confidence intervals based on 5 replicates are shown. (C) Growth curves of the reporter strains. The values represent the means and 95% confidence intervals of data for five replicate wells in a microtiter plate, where the cultures were grown and monitored.