TABLE 2.
Bacterial strains and plasmids used in this studya
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| JM105 | thi rpsL (Strr) endA sbcB15 sbcC hsdR4 (rK− mK−) Δ(lac-proAB) [F′ traD36 lacIq Δ(lacZ)M15 proA+B+] | Laboratory collection |
| JBAMG134 | JM105/pJBA285; GENr | Jens Bo Andersen |
| GZA42 | JM105/pGZA1; GENr | This study |
| T121 | DH5α/miniCTX2T2.1.-GW::Ptrc-mCherry; TETr | 20 |
| P. aeruginosa | ||
| PAO1 | Wild-type P. aeruginosa strain | 17 |
| PAONB (PAOΔnfxB) | PAO1 ΔnfxB::lox; constitutively expresses the MexCD-OprJ pump | 16 |
| PAO1-mCherry | PAO1 with mCherry integrated at the attB site via a mini-CTX vector; TETr marker removed via Flp-mediated recombination | This study |
| PAOΔnfxB-mCherry | PAOΔnfxB with mCherry integrated at the attB site via a mini-CTX vector; TETr marker removed via Flp-mediated recombination | This study |
| PAO1-mCherry-PCD-gfp+ | PAO1-mCherry with mini-Tn7 carrying the transcriptional fusion of PCD-gfp+; GENr | This study |
| PAOΔnfxB-mCherry-PCD-gfp+ | PAOΔnfxB-mCherry with mini-Tn7 carrying the transcriptional fusion of PCD-gfp+; GENr | This study |
| Plasmids | ||
| Mini-Tn7-PCD-lux | pUC18-mini-Tn7T-Gm-PCD-lux; GENr | 15 |
| pUX-BF13 | Helper plasmid with transposase for integration of the mini-Tn7 element; AMPr | 37 |
| pHA51 | Mini-CTX2T2.1.-GW::Ptrc-mCherry; TETr | 20 |
| pFLP2 | Site-specific excision vector with Flp recombinase and sacB; AMPr CARr | 38 |
| pJBA285 | Donor plasmid for RBSII-gfp+-T0; GENr | Jens Bo Andersen |
| pGZA1 | pUC18-mini-Tn7T-Gm-PCD-gfp+; GENr | This study |
a
AMPr, ampicillin resistance; CARr carbenicillin resistance; GENr, gentamicin resistance; TETr, tetracycline resistance; RBSII, strong ribosomal binding site; T0, transcriptional terminator derived from phage λ; PCD, mexCD-oprJ promoter with an NfxB binding site.