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. 2017 Feb 23;61(3):e02292-16. doi: 10.1128/AAC.02292-16

TABLE 3.

Primers used in this study

Primer Sequence (5′–3′) Description
RBSII-gfp(up)-fw ATATACCCGGGTCTAGAATTAAAGAGGAGAAATTAAGCATG RBSII-gfp-T0 amplification (forward)
Gfp(down)-rev TATATGGTACCCTCCTGAAAATCTCGCCAAGCTAGC RBSII-gfp-T0 amplification (reverse)
Pmexcd_fw TTGCTGTTGACAAAGGGAATC Sequencing
Gfp_fw_1 CAGTGGAGAGGGTGAAGGTGA Sequencing
Gfp-To(rev) new1 TCCTGAAAATCTCGCCAAGC Sequencing
Gfp_(rev) CCATAACCGAAAGTAGTGACAAGTG Sequencing
nfxB fw3 TGACACACCCGACCGTTG nfxB amplification and sequencing
nfxB rev1 TCGGTCCGTGCCATGC nfxB amplification and sequencing
PglmS-down GCACATCGGCGACGTGCTCTC Verification of correct mini-Tn7 element integration
PTn7R CACAGCATAACTGGACTGATTTC Verification of correct mini-Tn7 element integration