TABLE 2.
Ribosomal mutations and MICs observed in L. pneumophila sequenced clones and reconstructed mutants
| L. pneumophila strain | Nucleotidic or amino acid change(s) |
MICa |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 23S rRNAb (no. of mutated copies/total no. of copies) | L4 | L22 | ERY | AZI | CLR | SPI | LIN | PRI | TEL | LZ | LVX | CIP | RIF | DOX | |
| Ery1 | G2057A (3/3) + A2059C (3/3) | G66D | — | 1,024 | 2,048 | 256 | 100,000 | 512 | 0.25 | >4 | 16 | 0.032 | 0.016 | 0.0005 | 2 |
| Ery2 | C2611G (3/3) | Q62R + G64E | — | 1,024 | 128 | 128 | 6,250 | 64 | 0.25 | 4 | 16 | 0.032 | 0.016 | 0.0005 | 2 |
| Ery3 | C2611T (3/3) | G66A | — | 1,024 | 32 | 256 | 6,250 | 32 | 0.25 | 1 | 32 | 0.032 | 0.016 | 0.0005 | 2 |
| Ery4 | A2058T (2/3) | G66A | K90M | 1,024 | 1,024 | 256 | 100,000 | >512 | 0.5 | >4 | 8 | 0.032 | 0.016 | 0.0005 | 2 |
| Ery5 | G2057A (3/3) + A2058C (2/3) + A2059G (1/3) | G66A | — | 1,024 | 2,048 | >256 | 100,000 | 512 | 0.5 | >4 | 8 | 0.032 | 0.016 | 0.0005 | 2 |
| Ery6 | C2611T (3/3) | G66D | — | 1,024 | 64 | 64 | 6,250 | 256 | 0.125 | 1 | 32 | 0.032 | 0.016 | 0.0005 | 2 |
| Azi1 | C2611T (3/3) | T65K + G66R | G91D | 1,024 | 256 | 256 | 25,000 | 64 | 1 | >4 | 32 | 0.032 | 0.016 | 0.0005 | 2 |
| Azi2 | A2058G (3/3) | — | — | 1,024 | 2,048 | 512 | 12,500 | 256 | 0.25 | 4 | 16 | 0.032 | 0.016 | 0.0005 | 2 |
| Azi3 | C2611T (3/3) | T65K + G66C | — | 2,048 | 256 | 256 | 25,000 | 32 | 0.25 | 4 | 32 | 0.032 | 0.016 | 0.0005 | 2 |
| Azi4 | C2611G (3/3) | del 63KG64 | — | 2,048 | 256 | 512 | 25,000 | 64 | 1 | >4 | 32 | 0.032 | 0.016 | 0.0005 | 2 |
| Azi5 | A2058G (3/3) | T65K | P87L + G91D | 1,024 | 1,024 | 256 | 25,000 | >512 | 1 | >4 | 16 | 0.032 | 0.016 | 0.0005 | 2 |
| Azi6 | A2058G (3/3) | — | — | 2,048 | 2,048 | 512 | 12,500 | >512 | 0.125 | >4 | 16 | 0.032 | 0.016 | 0.0005 | 2 |
| ΔdotA L1 | A2058G (3/3) | — | — | ≥1,024 | 1,024 | 256 | 12,500 | >512 | 0.125 | >4 | 16 | 0.032 | 0.016 | 0.0005 | 2 |
| ΔdotA L2 | A2058G (2/3) | — | — | ≥1,024 | 1,024 | 32 | 6,250 | >512 | 0.06 | >4 | 16 | 0.032 | 0.016 | 0.0005 | 2 |
| ΔdotA L3 | G2057T (3/3) | — | — | 16 | 32 | 4 | 780 | 64 | 0.125 | 1 | 32 | 0.032 | 0.016 | 0.0005 | 2 |
| ΔdotA L4 | C2611T (2/3) | — | — | 256 | 8 | 4 | 1,560 | 64 | 0.25 | 1 | 8 | 0.032 | 0.016 | 0.0005 | 2 |
| ΔdotA L5 | C2611G (3/3) | — | — | 128 | 8 | 4 | 1,560 | 64 | 0.25 | 1 | 16 | 0.032 | 0.016 | 0.0005 | 2 |
| ΔdotA L6 | — | G66R | — | 8 | 4 | 0.063 | 1,560 | 64 | 0.25 | 0.25 | 16 | 0.032 | 0.016 | 0.001 | 2 |
| ΔdotA L7 | — | G66A | — | 4 | 2 | 0.063 | 1,560 | 64 | 0.25 | 0.25 | 16 | 0.032 | 0.016 | 0.001 | 2 |
| Paris WT | — | — | — | 0.25 | 0.25 | 0.063 | 390 | 64 | 0.125 | 0.125 | 16 | 0.032 | 0.016 | 0.0005 | 2 |
| Paris ΔdotA | — | — | — | 0.25 | 0.25 | 0.063 | 390 | 64 | 0.125 | 0.125 | 16 | 0.032 | 0.016 | 0.001 | 2 |
a
All MIC data represent milligrams per liter, except the spiramycin data, which represent IU per milliliter. ERY, erythromycin; AZI, azithromycin; CLR, clarithromycin; SPI, spiramycin; LIN, lincomycin; PRI, pristinamycin; TEL, telithromycin; LZ, linezolid; LVX, levofloxacin; CIP, ciprofloxacin; RIF, rifampin; DOX, doxycycline.
b
E. coli numbering. —, no mutation.