TABLE 1.

Impact of the N¹³²G substitution on kinetic parameters of avibactam and clavulanate and the inhibition of KPC-2 and CTX-M-15

β-Lactamase	Avibactam			Clavulanate
	k2/Ki (M−1 s−1)	k−2 (s−1)	koff (s−1)	kcat (s−1)	Km (μM)	kcat/Km (M−1 s−1)
KPC-2	(2.1 ± 1.0) × 105	(1.6 ± 0.4) × 10−3	(1.0 ± 0.6) × 10−3	5.2 ± 0.7	36 ± 4	(1.4 ± 0.3) × 105
KPC-2 N132G	53 ± 3	(1.1 ± 0.1) × 10−3	(1.8 ± 1.5) × 10−3	(1.5 ± 0.2) × 10−4	0.35 ± 0.05	430 ± 80
CTX-M-15	(2.1 ± 0.1) × 105	(1.6 ± 0.3) × 10−3	(6.7 ± 1.1) × 10−4	(3.0 ± 0.2) × 10−4	0.032 ± 0.005	(9.4 ± 1.6) × 103
CTX-M-15 N132G	70 ± 6	(2.2 ± 1.7) × 10−4	(3.2 ± 1.7) × 10−5	(1.2 ± 0.1) × 10−4	17 ± 2	7.1 ± 1.0