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. 2017 Feb 22;10:1091–1100. doi: 10.2147/OTT.S125724

Figure 3.

Figure 3

miR-622 targeting regulated DYRK2 in CRC cells. (A) DYRK2 was a target gene of miR-622 predicted by miRanda and targetscan. (B) Diagram of the luciferase reporter plasmids with the wild-type or mutant DYRK2 3′UTR. (C, D) The expression of DYRK2 and the activity of its downstream snailp-Ser104 was obviously decreased when transfection of miR-622 mimics, elucidated by Western blot and real time PCR. (E, F) When transfection of miR-622 inhibitor, DYRK2 and snailp-Ser104 was increased. (G, H) The relative luciferase activity in SW1116 and SW480 cells was determined by fluorescence changes after co-transfection of miR-622 mimics and the plasmid with wild-type or mutant DYRK2 3′UTR. Three independent experiments were performed in duplicate. All the data mentioned are shown as mean ± standard deviation. **P<0.01 vs control group.

Abbreviations: miR-622, microRNA-622; DYRK2, dual specificity tyrosine phosphorylation-regulated kinase 2; 3′UTR, 3′-untranslated region; CRC, colorectal cancer; PCR, polymerase chain reaction; mut, mutant; wt, wild-type; NC, negative control.