Table 1.

Cell densities, suspension volumes, and freezing rates for hPSC-CM cryopreservation

Reference	Cell density (cells/mL)	Freezing volume (mL)	Recovery rate (%), assay	Freezing rate (Δ°C/min)
Xu et. al. [46]	~ 27 million	1.5	70–77, cell counting and flow cytometry	1 until −40°C
	~ 53 million	1.5		5 from −40°C to −80°C
	40 million	0.25
Zhu et.al. [39]	40 million	0.25	Not reported	1 from 0°C to −7°C
				0.75 from −7°C to −10°C
				1 from −10°C to −80°C
Chen et.al. [49]	10 million	1	84.3 ± 5.2, trypan blue exclusion	Controlled rate program not specified
	15 million	2