FIGURE 1:

Localization of Ede1-GFP truncation mutants. (A) Schematic diagram of full-length Ede1 and the truncation constructs that were integrated at the EDE1 locus. A C-terminal GFP tag, present on all constructs, is not shown. (B) Representative images from the first frame of movies of cells expressing the indicated constructs imaged in the same field as Ede1-GFP reference cells labeled with FM4-64 (magenta). Movies of the GFP channel were taken at 2 s/frame for 4 min, immediately followed by capture of a still image of FM4-64. Scale bar, 5 μm. (C) Magnification of the cell outlined in a white box from B followed by circular kymographs of the mother and bud cortex. Scale bars, 2 μm and 20 s.