FIGURE 3:

ede1^EH3PPCConly and ede1^ΔCCinternal facilitate endocytic initiation but not normal maturation timing. (A) The 90-s movies of Sla1-GFP in cells expressing 13xmyc-tagged Ede1 truncations were taken at 1 s/frame. First frame from a representative cell. Scale bar, 5 μm. This is followed by circular kymographs of the bud and mother cortex of the cells shown in A; scale bars, 2 μm and 20 s. (B) Quantification of kymograph analysis from three independent experiments of the number of patches that start during a 90-s movie in cells of the indicated genotype. The p values were calculated using Student’s t test. (C) Quantification of kymograph analysis from three independent experiments of the proportion of Sla1-GFP patches that internalize per cell (25 cells/strain). (D) Quantification of the lifetime of Sla1-GFP from three independent experiments in cells of the indicated genotype. (E) ede1^ΔCCinternal-GFP and Sla1-mCherry were imaged for 4 min at 2 s/frame with ∼300-ms delay between channels. The first frame of a movie of a representative cell, in addition to a circular kymograph of the bud and mother cortex. Scale bars, 2 μm and 40 s.