Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Mar 1;28(5):624–633. doi: 10.1091/mbc.E16-09-0648

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Funabashi, Katoh, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 1: — Interaction of KIF17 with the IFT46–IFT56 dimer demonstrated by the VIP assay. (A) Interaction of KIF17 with the IFT-B core subcomplex. Lysates were prepared from HEK293T cells coexpressing EGFP-KIF17 and all IFT-B subunits, all core subunits, or all peripheral subunits fused to mChe/tRFP and processed for the VIP assay using GST-tagged anti-GFP Nb as described in Materials and Methods. (B) A subtractive VIP assay was performed to determine the IFT-B core subunits involved in its interaction with KIF17. Lysates prepared from HEK293T cells coexpressing EGFP-KIF17 and all but one (as indicated) of the core subunits fused to mChe/tRFP were processed for the VIP assay. (C, D) Confirmation of the involvement of the IFT46–IFT56 dimer in the KIF17–IFT-B interaction. Lysates prepared from HEK293T cells coexpressing EGFP-KIF17 and mChe-tagged IFT46 (lane 1), IFT52 (lane 2), IFT56 (lane 3), IFT46 + IFT52 (lane 4), IFT46 + IFT56 (lane 5), or IFT46 + IFT52 + IFT56 (lane 6) were processed for the VIP assay (C) or immunoblotting analysis (D) as described in Materials and Methods. (E) Schematic representation of the interaction of KIF17 with the IFT46–IFT56 dimer in the IFT-B complex.