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. 2017 Mar 1;28(5):634–644. doi: 10.1091/mbc.E16-10-0750

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© 2017 Shukla et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — EB1 is a good indicator of MT dynamics. (A) Localization of EB1-mRFP (grayscale, inverted) and TubA-GFP (green in montage) from Supplemental Video S1 (strain NS133) showing dynamics of EB1 mRFP comets at the plus ends of growing MTs. (B) EB1-CR and the GcpC-GFP SPB marker during mitosis (Supplemental Video S2, strain NS327). EB1-CR localizes to the mitotic spindle and then to astral MTs during mitotic exit (inset, blue arrows). (C) EB1-GFP (strain NS326) disperses from comets when MTs are depolymerized using benomyl and reappears at mobile comets after benomyl washout. EB1-GFP comets reemerge from SPBs (marked by Ndc80), septa (pink arrow), and cytoplasmic foci (blue arrows). Scale bar, 10 μm.