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. 2017 Mar 1;28(5):634–644. doi: 10.1091/mbc.E16-10-0750

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© 2017 Shukla et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Treatment with the H₂S donor NaHS reversibly depolymerizes MTs. (A) After treatment with 100 μM NaHS, EB1-GFP (strain NS326) initially disperses from comets but then locates to fibrous and bar-like structures (Supplemental Video S8). (B) After washout, EB1-GFP rapidly returned to comets, indicating immediate MT repolymerization (Supplemental Video S9). (C) ClipA-GFP (strain NS141) also dispersed and then located to bar-like structures upon NaHS addition (Supplemental Video S10). (D) ClipA-GFP also relocated to mobile comets immediately after washout (Supplemental Video S11). (E) On NaHS treatment, NudA-3XGFP (strain NS329) rapidly transitioned into fibrous and bar-like structures (Supplemental Video S12) and it also (F) resumed its dynamic behavior after NaHS washout (Supplemental Video S13). Scale bar, 10 μm.