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. Author manuscript; available in PMC: 2017 Feb 27.
Published in final edited form as: J Biol Chem. 2004 Oct 1;279(49):51075–51081. doi: 10.1074/jbc.M408607200

Fig. 3. Lack of GSK-3β does not enhance UV-induced JNK phosphorylation.

Fig. 3

GSK-3β−/− and GSK-3β+/+ MEFs were subjected to UV light radiation at the indicated doses (J, J/m2) using a UV light cross-linker as described under “Experimental Procedures.” Following UV light exposure, the cells were re-fed serum-free medium and returned to a CO2 incubator for 30 min. Cells were then lysed in SDS sample buffer and analyzed for JNK phosphorylation by immunoblotting with a JNK phospho-specific (p-JNK) antibody. Reprobing with anti-JNK1 antibody was included to confirm comparable levels of loading among samples.