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. Author manuscript; available in PMC: 2017 Feb 27.
Published in final edited form as: J Biol Chem. 2004 Oct 1;279(49):51075–51081. doi: 10.1074/jbc.M408607200

Fig. 5. Increased JNK activation in GSK-3β−/− cells does not induce apoptosis and is associated with an increase in mitogenic response to LPA.

Fig. 5

A, GSK-3β−/− and GSK-3β+/+ cells were starved and incubated without or with LPA (10 μM). After 24 h, cells (including floating and adherent cells) were harvested, fixed, and stained via terminal deoxynucleotidyltransferase-mediated dUTP nick end-labeling (TUNEL) using ApopTag (Chemicon International). The percentages of TUNEL-stained positive apoptotic cells were determined by flow cytometry. The results are presented as means ± S.D. of duplicate data from three independent experiments. B, the mitogenic activity of LPA in GSK-3β−/− and GSK-3β+/+ cells was measured by [3H]thymidine incorporation as described under “Experimental Procedures.” The data were presented as fold increases (means ± S.D. of triplicate assays) with the activity in unstimulated control cells defined as one.