Fig. 5.

Data on Emodin increased cell viability and reduced apoptosis of HUVECs after gamma irradiation (24Gy). (A)Improvement in the HUVECs survival rate after irradiation in the emodin-treated group (10, 1, and 0.1 µM). (B)and(D) Annexin V/PI double staining assessed using flow cytometry. (C)and(E)Hoechst 33258 staining was performed to evaluate apoptosis in the HUVECs. Emodin was added at 24 h prior to exposure to radiation, then both cell viability and cell apoptosis were analyzed at 24 h post-irradiation. Values are presented as the mean±SEM (n=3;^#p<0.05,^##p<0.01, ^###p<0.001,control group vs. radiation group; ^*p<0.05,^**p<0.01, emodin treatment group vs .radiation group).