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Fluorescent images of the wild type TUBB4A and HLD6-assocated mutants (Arg2-to-Glu, Arg156-to-Leu, and Thr178-to-Arg). Cells were transfected with the plasmids encoding GFP-tagged TUBB4A and the mutants. Representative images (left panels) of GFP-tagged, wild type TUBB4A (with fiber structures) and mutants (without fiber structures) are shown. Fluorescence intensity of lanes 1 (blue) and 2 (red) is also shown in right panels. Scale bar indicates 10 μm.
